Reliability of plasma lipopolysaccharide-binding protein (LBP) from repeated measures in healthy adults

Abstract

Plasma lipopolysaccharide-binding protein (LBP), a measure of internal exposure to bacterial lipopolysaccharide, has been associated with several chronic conditions and may be a marker of chronic inflammation; however, no studies have examined the reliability of this biomarker in a healthy population. We examined the temporal reliability of LBP measured in archived samples from participants in two studies. In Study one, 60 healthy participants had blood drawn at two time points: baseline and follow-up (either three, six, or nine months). In Study two, 24 individuals had blood drawn three to four times over a seven-month period. We measured LBP in archived plasma by ELISA. Test–retest reliability was estimated by calculating the intraclass correlation coefficient (ICC). Plasma LBP concentrations showed moderate reliability in Study one (ICC 0.60, 95 % CI 0.43–0.75) and Study two (ICC 0.46, 95 % CI 0.26–0.69). Restricting the follow-up period improved reliability. In Study one, the reliability of LBP over a three-month period was 0.68 (95 % CI: 0.41–0.87). In Study two, the ICC of samples taken ≤seven days apart was 0.61 (95 % CI 0.29–0.86). Plasma LBP concentrations demonstrated moderate test–retest reliability in healthy individuals with reliability improving over a shorter follow-up period.

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