MicroRNA-21 regulates the ERK/NF-κB signaling pathway to affect the proliferation, migration and apoptosis of human melanoma A375 cells by targeting SPRY1, PDCD4 and PTEN

ABSTRACT

Objective

To explore the effects of microRNA-21 (miR-21) and ERK/NF-κB signaling pathway on human melanoma A375 cells.

Methods

The melanoma tissues and adjacent normal tissues were obtained from 45 melanoma patients. qRT-PCR was conducted to quantify the expression of miR-21 and the gene mRNA expressions. Human melanoma A375 cells were divided into the Mock, negative control (NC), miR-21 inhibitors, miR-21 inhibitors + siRNA-SPRY1, miR-21 inhibitors + siRNA-PDCD4 and miR-21 inhibitors + siRNA-PTEN groups. Western blotting was used to determine protein expressions. CCK8 assay and Transwell assay were performed to evaluate the proliferation, migration and invasion of A375 cells. Annexin V/propidium iodide double staining and flow cytometry were adopted to detect cell apoptosis.

Results

MiR-21 expression was higher in melanoma tissues than in adjacent tissues, while the mRNA and protein expressions of SPRY1, PDCD4 and PTEN were lower in melanoma tissues than in adjacent tissues. Compared with the Mock and NC groups, the miR-21 inhibitors group exhibited increased expressions of SPRY1, PDCD4 and PTEN and decreased expressions of ERK, p-ERK, NF-κB p65 and p-NF-κB p65. After transfection of miR-21 inhibitors, the proliferation, migration and invasion of A375 cells were inhibited, while the apoptosis of A375 cells was promoted. However, the effects of miR-21 inhibitors on the growth, migration, invasion and apoptosis of A375 cells were reversed after transfection of siRNA-SPRY1, siRNA-PDCD4 or siRNA-PTEN.

Conclusion

MiR-21 can promote the proliferation, migration and inhibit the apoptosis of human melanoma A375 cells by inhibiting SPRY1, PDCD4 and PTEN via ERK/NF-κB signaling pathway. This article is protected by copyright. All rights reserved

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