The features of adipose-derived stem cells in patients with inflammatory bowel diseases

Abstract

Purpose

The treatment outcomes for inflammatory bowel disease (IBD) have been improving, but the development of better therapies is needed. Stem cell therapy is promising, but little is known about the differences in adipose-derived stem cells (ADSCs) between IBD patients and healthy individuals.

Methods

ADSCs were isolated from subcutaneous adipose tissue (SAT) in IBD (Crohn's disease, 3; ulcerative colitis, 2) and non-IBD (colorectal cancer, 5; breast cancer, 1) patients. We also analyzed the effects of tumor necrosis factor (TNF)-α on murine ADSCs.

Results

The numbers of stromal vascular fraction (SVF) cells per gram of SAT were 7.72 ± 3.03 × 10⁵ in IBD and 8.51 ± 8.80 × 10⁵ in non-IBD patients (p > 0.05). The proportions of ADSCs in SVF cells were 4.98 ± 2.61% in IBD and 1.02 ± 0.67% in non-IBD patients. The numbers of ADSCs per gram of SAT were 4.16 ± 2.96 × 10⁴ in IBD and 0.88 ± 1.04 × 10⁴ in non-IBD patients. The proportions and numbers of ADSCs were significantly higher in IBD patients than in non-IBD patients. TNF-α significantly facilitated the proliferation and motility of murine ADSCs.

Conclusion

These results showed the potential advantage of freshly isolated autologous ADSCs in IBD patients.

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