LncRNA RP11-552M11.4 promotes cells proliferation, migration and invasion by targeting BRCA2 in ovarian cancer

Summary

This study aimed to investigate the effect of long non-coding RNA (lncRNA) RP11-552M11.4 on cells proliferation, apoptosis, migration and invasion as well as its targeting genes in epithelial ovarian cancer (EOC) cells. LncRNA RP11-552M11.4 expression was detected in 67 tumor tissues and paired adjacent tissues obtained from EOC patients. lncRNA RP11-552M11.4 mimic/inhibitor plasmids were transferred into ovarian cancer cells (SKOV3, A-2780) and normal ovarian epithelial cells (IOSE80 cells). In addition, rescue experiment was performed by transferring BRCA2 inhibitor&lncRNA RP11-552M11.4 inhibitor plasmids into SKOV3 and A-2780 cells. qPCR, western blot, CKK-8, AV/PI, wound-healing and matrigel invasion assays were performed to detect RNA expression, protein expression, cells proliferation, apoptosis, migration and invasion respectively. LncRNA RP11-552M11.4 expression was elevated in tumor tissues compared with paired adjacent tissues and correlated with higher pathological grade, FIGO stage and worse overall survival in EOC patients. LncRNA RP11-552M11.4 promoted SKOV3 cells proliferation, migration and invasion while inhibited the apoptosis. Rescue experiment and luciferase reporter assay revealed that lncRNA RP11-552M11.4 regulated SKOV3 cells functions via binding BRCA2. Further experiments in A-2780 cells also validated that lncRNA RP11-552M11.4 induced A-2780 cells proliferation while repressed apoptosis by targeting BRCA2. In addition, upregulation of lncRNA RP11-552M11.4 increased IOSE80 cells proliferation, migration and invasion while decreased apoptosis. In conclusion, lncRNA RP11-552M11.4 correlates with worse prognosis, and promotes cells proliferation, migration, invasion and inhibits cells apoptosis by downregulating BRCA2 in EOC.

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