Itraconazole-induced inhibition on human esophageal cancer cell growth requires AMPK activation

We here evaluated the anti-esophageal cancer cell activity by the antifungal drug itraconazole. Our results show that μg/mL concentrations of itraconazole potently inhibited survival and proliferation of established (TE-1 and Eca-109) and primary human esophageal cancer cells. Itraconazole activated AMP-activated protein kinase (AMPK) signaling, which was required for subsequent esophageal cancer cell death. Pharmacological AMPK inhibition, AMPKα1 shRNA or dominant negative mutation (T172A) almost completely abolished itraconazole-induced cytotoxicity against esophageal cancer cells. Significantly, itraconazole induced AMPK-dependent autophagic cell death (but not apoptosis) in esophageal cancer cells. Further, AMPK activation by itraconazole induced multiple receptor tyrosine kinases (RTKs: EGFR, PDGFRα and PDGFRβ) lysosomal translocation and degradation to inhibit downstream Akt activation. In vivo, itraconazole oral gavage potently inhibited Eca-109 tumor growth in severe combined immunodeficient (SCID) mice. It was yet ineffective against AMPKα1 shRNA-expressing Eca-109 tumors. The in vivo growth of the primary human esophageal cancer cells was also significantly inhibited by itraconazole administration. AMPK activation, RTKs degradation and Akt inhibition were observed in itraconazole-treated tumors. Together, itraconazole inhibits esophageal cancer cell growth via activating AMPK signaling.

from Cancer via ola Kala on Inoreader https://ift.tt/2pPvtsk
via IFTTT