Δευτέρα 8 Αυγούστου 2016

Peperomin E reactivates silenced tumor suppressor genes in lung cancer cells via inhibition of DNA methyltransferase

Summary

Advanced lung cancer has poor prognosis owing to its low sensitivity to current chemotherapy agents. Therefore, discovery of new therapeutic agents is urgently needed. In this study, we investigated the antitumor effects of peperomin E, a secolignan isolated from Peperomia dindygulensis, a frequently used Chinese folk medicine for lung cancer treatment. The results indicate that peperomin E exhibits antiproliferative effects, promoting apoptosis and cell-cycle arrest in non-small cell lung cancer (NSCLC) cell lines in a dose-dependent manner, while demonstrating lower toxicity against normal human lung epidermal cells. Peperomin E inhibited tumor growth in A549 xenograft BALB/c nude mice without significant secondary adverse effects, indicating that it may be safely used to treat NSCLC. Furthermore, the mechanisms underlying the anticancer effects of peperomin E have been investigated. Using an in silico target fishing method, we observed that peperomin E directly interacts with the active domain of DNA methyltransferase 1 (DNMT1), potentially affecting its genome methylation activity. Subsequent experiments verified that peperomin E decreased DNMT1 activity and expression, thereby decreasing global methylation and reactivating the epigenetically silenced tumor suppressor genes (TSGs) including RASSF1A, APC, RUNX3 and p16INK4, which in turn activates their mediated pro-apoptotic and cell-cycle regulate signaling pathways in lung cancer cells. The observations herein report for the first time that peperomin E is a potential chemotherapeutic agent for NSCLC. The anticancer effects of peperomin E may be partly attributable to its ability to demethylate and reactivate methylation-silenced TSGs through direct inhibition of the activity and expression of DNMT1.



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