A Novel Concept for Surgical Management of a Traumatic Comminuted Cricoid Fracture

xlomafota13 shared this article with you from Inoreader A Novel Concept for Surgical Management of a Traumatic Comminuted Cricoid Fracture Via ear nose throat Ear Nose Throat J. 2021 Sep 1:1455613211040579. doi: 10.1177/01455613211040579. Online ahead of print. ABSTRACT The cricoid plays 2 key roles: phonation and maintenance of the airway frame, both of which are lost in cases of comminuted cricoid fractures. The management of these 2 functions becomes a challenge in planning surgical treatment. We report the treatment course in a case of traumatic comminuted cricoid fracture that was resolved with good airway and phonatory funct ions. A 25-year-old man fell down the stairs and complained of respiratory discomfort and hoarseness of voice. A computed tomography scan showed comminuted cricoid fracture; therefore, surgery was performed to restore the patient's airway and phonation functions. We found that the airway was maintained by the anterior part and that the phonation depended on the posterior part of the cricoid. This novel concept helped clarify the treatment goal in this case of comminuted cricoid fractures. Furthermore, it is important that the anterior part of the cricoid is reconstructed with sufficient internal diameter, while the posterior part of the cricoid is reconstructed in the correct position. PMID:34467797 | DOI:10.1177/01455613211040579 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Role of lncRNA BCYRN1 in trophoblast cell physiology and pathogenesis of preeclampsia

xlomafota13 shared this article with you from Inoreader Role of lncRNA BCYRN1 in trophoblast cell physiology and pathogenesis of preeclampsia Via Experimental and therapeutic medicine Exp Ther Med. 2021 Oct;22(4):1137. doi: 10.3892/etm.2021.10571. Epub 2021 Aug 6. ABSTRACT Long non-coding RNAs (lncRNAs) may play a key role in the pathogenesis of preeclampsia (PE). The present study investigated the role of the lncRNA brain cytoplasmic RNA 1 (BCYRN1) in PE. A total of 30 patients with severe PE (SPE) and 30 patients with mild PE (MPE) were recruited, whilst 30 healthy pregnant individuals were enrolled as controls. Placental tissues of enrolled subjects were collected after delivery. The clinical data of pregnant women and newborns were recorded before the correlation between BCYRN1 expression and clinical characteristics was analyzed. Furthermore, HTR-8/SVneo cells were transfected with BCYRN1 overexpression plasmids and BCYRN1 small interfering (si)RNA. Cell Counting Kit-8, Transwell, flow cytometry and tube formation assays were used to detect the function of BCYRN1 in HTR-8/SVneo cells. Reverse transcription-quan titative PCR was used to detect BCYRN1 expression in placental tissues and HTR-8/SVneo cells. Western blotting was used to detect the protein expression levels of Wnt1 and β-catenin. BCYRN1 expression was lower in placenta with mild PE compared with in normal placenta, and was in turn lower in placenta with severe PE. BCYRN1 was negatively correlated with systolic blood pressure and 24-h urinary protein in patients with PE. BCYRN1 siRNA inhibited cell viability, migration, invasion and tube forming abilities whilst increasing apoptosis. By contrast, BCYRN1 overexpression conferred opposite effects. The levels of Wnt1 and β-catenin expression in the cells and placental tissues were next measured. Cells overexpressing BCYRN1 were further treated with the Wnt pathway inhibitor XAV939. Wnt1 and β-catenin expression were elevated when BCYRN1 was overexpressed, but were decreased after BCYRN1 knockdown. XAV939 attenuated the effect of BCYRN1 overexpression on HTR-8/SVneo cells. Overall , the resulted indicated that upregulation of BCYRN1 increased trophoblast viability and prevented apoptosis by activating the Wnt/β-catenin pathway to delay PE onset. PMID:34466147 | PMC:PMC8383326 | DOI:10.3892/etm.2021.10571 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

SNAI2 is induced by transforming growth factor-β1, but is not essential for epithelial-mesenchymal transition in human keratinocyte HaCaT cells

xlomafota13 shared this article with you from Inoreader SNAI2 is induced by transforming growth factor-β1, but is not essential for epithelial-mesenchymal transition in human keratinocyte HaCaT cells Via Experimental and therapeutic medicine Exp Ther Med. 2021 Oct;22(4):1124. doi: 10.3892/etm.2021.10558. Epub 2021 Aug 4. ABSTRACT Epithelial-mesenchymal transition (EMT) is a cellular process in which epithelial cells lose their epithelial traits and shift to the mesenchymal phenotype, and is associated with various biological events, such as embryogenesis, wound healing and cancer progression. The transcriptional program that promotes phenotype switching is dynamically controlled by transcription factors during EMT, including Snail (SNAI1), twist family bHLH transcription factor (TWIST) and zinc finger E-box binding homeobox 1 (ZEB1). The present study aimed to investigate the molecular mechanisms underlying EMT in squamous epithelial cells. Western blot analysis and immunocytochemical staining identified Slug (SNAI2) as a transcription factor that is induced during transforming growth factor (TGF)-β1-mediated EMT in the human keratinocyte cell line HaCaT. The effect of SN AI2 overexpression and knockdown on the phenotypic characteristics of HaCaT cells was evaluated. Filamentous actin staining and western blot analysis revealed that the overexpression of SNAI2 did not induce the observed EMT-related phenotypic changes. In addition, SNAI2 knockdown demonstrated almost no impact on the EMT phenotypes induced by TGF-β1. Notably, DNA microarray analysis followed by comprehensive bioinformatics analysis revealed that the differentially expressed genes upregulated by TGF-β1 were significantly enriched in cell adhesion and extracellular matrix binding, whereas the genes downregulated in response to TGF-β1 were significantly enriched in the cell cycle. No enriched gene ontology term and biological pathways were identified in the differentially expressed gene sets of SNAI2-overexpressing cells. In addition, the candidates for master transcription factors regulating the TGF-β1-induced EMT were identified using transcription factor enrichment analysis. In c onclusion, the results of study demonstrated that SNAI2 does not play an essential role in the EMT of HaCaT cells and identified candidate transcription factors that may be involved in EMT-related gene expression induced by TGF-β1. These findings may enhance the understanding of molecular events in EMT and contribute to the development of a novel therapeutic approach against EMT in cancers and wound healing. PMID:34466140 | PMC:PMC8383325 | DOI:10.3892/etm.2021.10558 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Tumor microenvironment is not an 'innocent bystander' in the resistance to treatment of head and neck cancers (Review)

xlomafota13 shared this article with you from Inoreader Tumor microenvironment is not an 'innocent bystander' in the resistance to treatment of head and neck cancers (Review) Via Experimental and therapeutic medicine Exp Ther Med. 2021 Oct;22(4):1128. doi: 10.3892/etm.2021.10562. Epub 2021 Aug 5. ABSTRACT Head and neck cancers are still one of the most common types of cancer in the world. They rank in the leading sixth place in terms of incidence globally, and the incidence continues to rise. The mortality rates remain at high levels. Pathological subclassification places squamous cell carcinoma of the head and neck (HNSCC) in the first place concerning the histological forms of head and neck cancers; a tumor with extremely aggressive behavior and high mortality rates. The tumor microenvironment is a very complex ecosystem of cellular and non-cellular components, characterized by unique features, that contribute to the appearance of immunosuppression and diminished anticancer immunity, impacting patient prognosis and treatment outcome. Despite many important advances in therapy, resistance to therapy represents a difficult challenge in HNSCC patien ts. Tumor progression, metastasis, and response to therapy are all influenced by the complex ecosystem represented by the tumor microenvironment and by the interactions between cellular and non-cellular components of this system. Therefore, the tumor microenvironment, in the light of recent data, is not an innocent bystander. In the last few years, there has been a sustained effort to characterize the tumor microenvironment, to identify targets of response and identify other mechanisms of tumor-specific immune responses, or to discover other biomarkers of response. There is an urgent need to understand how to properly select patients, the therapy sequence, and how to use feasible biomarkers that can help to identify the patient who may obtain the most benefit from available therapies. PMID:34466142 | PMC:PMC8383332 | DOI:10.3892/etm.2021.10562 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

MicroRNA-9 inhibits the proliferation and migration of malignant melanoma cells via targeting sirituin 1

xlomafota13 shared this article with you from Inoreader MicroRNA-9 inhibits the proliferation and migration of malignant melanoma cells via targeting sirituin 1 Via Experimental and therapeutic medicine Exp Ther Med. 2021 Oct;22(4):1135. doi: 10.3892/etm.2021.10569. Epub 2021 Aug 6. ABSTRACT [This retracts the article DOI: 10.3892/etm.2017.4595.]. PMID:34466145 | PMC:PMC8383330 | DOI:10.3892/etm.2021.10569 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Korsakoff syndrome: An overlook (Review)

xlomafota13 shared this article with you from Inoreader Korsakoff syndrome: An overlook (Review) Via Experimental and therapeutic medicine Exp Ther Med. 2021 Oct;22(4):1132. doi: 10.3892/etm.2021.10566. Epub 2021 Aug 5. ABSTRACT This review aimed to analyze the latest neurobiological findings regarding Korsakoff syndrome, since alcoholism is the most prevalent addiction worldwide. In addition, we analyzed the optimal treatment that can be administered in order to minimize the symptoms and improve the outcome of these patients. The disruption of memory circuits within the brain of alcoholic patients results in the amnestic syndrome known as Korsakoff syndrome. It is generally characterized by a chronic neuropsychiatric syndrome caused by vitamin B1 (thiamine) deficiency. Other categories of patients can develop Korsakoff syndrome without consuming alcohol such as AIDS patients, terminally ill cancer patients, or patients with chronic infections and malnutrition. Vitamin B1 is required in the Krebs cycle for production of adenosine triphosphate (ATP). It is also a cofactor in the production of acetylcholine and certain neurotransmitters. Alcohol consumption can decrease the intake, gastrointestinal absorption and cellular utilization of vitamin B1. Treatment of alcohol withdrawal along with high doses of vitamin B1 can improve the general outcome of patients. A small percentage of patients can recover from Wernicke's encephalopathy with no permanent brain damage. The onset of Korsakoff syndrome darkens the prognosis. Alcohol abstinence is an absolute recommendation and prevents the extension of neural damage. PMID:34466144 | PMC:PMC8383329 | DOI:10.3892/etm.2021.10566 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

lncRNA and mRNA sequencing of the left testis in experimental varicocele rats treated with Morinda officinalis polysaccharide

xlomafota13 shared this article with you from Inoreader lncRNA and mRNA sequencing of the left testis in experimental varicocele rats treated with <em>Morinda officinalis</em> polysaccharide Via Experimental and therapeutic medicine Exp Ther Med. 2021 Oct;22(4):1136. doi: 10.3892/etm.2021.10570. Epub 2021 Aug 6. ABSTRACT Varicocele is a common disease of the male reproductive system. Morinda (M.) officinalis is a Chinese herbal medicine, whose main bioactive component M. officinalis polysaccharide (MOP) is believed to have a therapeutic effect on varicocele; however, the underlying molecular mechanisms of this effect are poorly understood. In the present study, 24 rats were randomly divided into three groups: i) Control group; ii) experimental varicocele group; and iii) 300 mg/kg MOP administration group. Analysis of mRNA and long non-coding RNA (lncRNA) expression in rat left testicular tissue was performed. The results suggested that a total of 144 mRNAs and 63 lncRNAs, 63 mRNAs and 148 lncRNAs, and 173 mRNAs and 54 lncRNAs were differentially expressed between the varicocele non-treatment and control groups, the varicocele treatment and varicocele non-treatment groups, and the varicocele treatment and control groups, respectively. Following validation by reverse transcription-quantitative PCR, the Yip1 domain family member 7 (YIPF7) gene was identified as a key mediator of varicocele pathogenesis and repair effect of MOP. Additionally, genes such as purinergic receptor P2X 4 (P2RX4), transmembrane protein 225B (TMEM255B) and Wnt family member 9B (WNT9B) were confirmed to be differentially expressed between the varicocele non-treatment and control groups. We hypothesize that TMEM255B could be a potential novel diagnostic biomarker for varicocele; WNT9B and P2RX4 likely play notable roles in the pathophysiology of the disease through the Wnt signaling pathway and regulation of transmembrane ion channels, respectively. In summary, the present study delineated the molecular mechanisms underlying varicocele pathogenesis and the therapeutic effect of MOP, identified a potential nove l diagnostic marker and therapeutic target for varicocele, and provided feasible directions for further studies in the future. PMID:34466146 | PMC:PMC8383328 | DOI:10.3892/etm.2021.10570 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Association between the levels of CGI-58 and lipoprotein lipase in the placenta of patients with preeclampsia

xlomafota13 shared this article with you from Inoreader Association between the levels of CGI-58 and lipoprotein lipase in the placenta of patients with preeclampsia Via Experimental and therapeutic medicine Exp Ther Med. 2021 Oct;22(4):1129. doi: 10.3892/etm.2021.10563. Epub 2021 Aug 5. ABSTRACT Preeclampsia is an idiopathic disease of pregnancy, which seriously endangers the life of both the mother and the infant. The pathogenesis of preeclampsia has not been fully elucidated, although it is generally considered to be associated with abnormal lipid metabolism during pregnancy. Comparative gene identification-58 (CGI-58) and lipoprotein lipase (LPL) are involved in the first step of triglyceride hydrolysis and serve an important role in lipid transport in the placenta. The present study aimed therefore to investigate the association between CGI-58 and LPL in the placentas of patients with or without preeclampsia and to evaluate blood lipid levels. The patient cohort was divided into two groups, pregnant women with preeclampsia and normal pregnant women (control). According to biochemical analyses, reverse transcription-quantitative PCR, i mmunohistochemistry analysis and western blotting, the expression of CGI-58 and LPL in the placenta was detected, the blood lipid levels were evaluated and other clinical data were collected. Compared with the control group, triglycerides (TGs), low density lipoprotein-cholesterol (LDL-C), apolipoprotein B (ApoB) and atherosclerotic index (AI) were significantly higher in the preeclampsia group, whereas high density lipoprotein-cholesterol (HDL-C) and apolipoprotein A (ApoA) were significantly lower (P<0.05). Furthermore, the expression levels of CGI-58 and LPL in the placental tissue of the preeclampsia group was significantly lower than that of the control group (P<0.05). Linear correlation analysis demonstrated that there was a positive association between CGI-58 and LPL (r=0.602; P<0.05), that CGI-58 was positively associated with HDL-C (r=0.63; P<0.01) but negatively associated with TG and ApoB (r=0.840; P<0.01; and r=0.514; P<0.05, respectively), that LPL was positively associated with HDL-C (r=0.524; P<0.01) but negatively associated with TG and AI (r=0.659; P<0.01; and r=0.496; P<0.01, respectively). These results suggested that the expression of CGI-58 and LPL in the placenta was associated with the pathogenesis of preeclampsia and maternal lipids and the risk of preeclampsia was increased with decreasing expression levels of CGI-58 and LPL. Hence, CGI-58 and LPL may be used as important indicators for the diagnosis of preeclampsia and for the prevention of preeclampsia in pregnant women. PMID:34466143 | PMC:PMC8383331 | DOI:10.3892/etm.2021.10563 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Effects of endurance exercise on serum inflammatory cytokine level and kidney structure in a rat diabetes model

xlomafota13 shared this article with you from Inoreader Effects of endurance exercise on serum inflammatory cytokine level and kidney structure in a rat diabetes model Via Experimental and therapeutic medicine Exp Ther Med. 2021 Oct;22(4):1125. doi: 10.3892/etm.2021.10559. Epub 2021 Aug 5. ABSTRACT Diabete mellitus (DM) is becoming a global health problem. Whilst many studies have previously focused on the therapeutic effects of exercise on diabetes, insufficient data exist on its effectiveness on disease prevention. The present study was designed to evaluate the effects of endurance exercises on kidney injury and on the expression of metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs) and transforming growth factor (TGF)-β1. Type 2 diabetic rat model was created followng 8 weeks of feeding on high fat diet, followed by injection with streptozotocin (STZ; 30 mg/kg). A total of three different intensity endurance exercises, including low-intensity exercise (8 m/min and 0˚ slope), moderate-intensity exercise (15 m/min and 5˚ slope) and high-intensity exercise (20 m/min and 10˚ slope), were arranged during this proces s. Oral glucose tolerance (OGTT) and oral sucrose tolerance tests (OSTT) were performed in all rats 1 week after STZ injection. Serum interleukin-6 and tumor necrosis factor-α levels were measured using ELISA. After OGTT, all rats were sacrificed and kidney samples were removed for hematoxylin and eosin staining and western blot analyzes. Urea and ureatinine levels, representative of renal function, were estimated by using automatic biochemical analyzer. Rats in the DM group showed severe impaired glucose tolerance, which was alleviated in the moderate-intensity exercise (ME) and the high-intensity exercise (HE) groups. Inflammatory cytokines were also significantly reduced rats in the ME group compared with those in the DM group. No difference in renal function, MMP-9/TIMP-1 and TGF-β1 expression was observed. In addition, rats in the DM group exhibited glomerular enlargement with structural renal abnormalities, whilst those in the ME and HE groups showed improved symptoms. To co nclude, no increased expression of inflammatory cytokines and renal fibrotic proteins could be observed in the present rat model of type-2 DM, but evident structural abnormalities can be observed in the kidneys. Medium-intensity endurance exercise can reduce serum inflammatory cytokine levels and prevent aberrant changes in renal structures. PMID:34466141 | PMC:PMC8383327 | DOI:10.3892/etm.2021.10559 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Management of a giant cervical lipoma: case report and literature review

xlomafota13 shared this article with you from Inoreader Management of a giant cervical lipoma: case report and literature review Via oto-rhino-laryngologie Pan Afr Med J. 2021 Jun 3;39:100. doi: 10.11604/pamj.2021.39.100.12727. eCollection 2021. ABSTRACT Lipoma is the most common of soft tissue tumours. It rarely occurs in the head and neck. Patients with fast-growing large sized lesion (> 10cm) should be suspected to have a cancer. We here report the case of a patient presenting with unusual cervical lipoma (size: approximately 46cm), diagnosed based on imaging tests, including computed tomography (CT) scan. Patient´s management was based on surgery. PMID:34466202 | PMC:PMC8379405 | DOI:10.11604/pamj.2021.39.100.12727 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.