Pepsinogen/Proton Pump Co‐Expression in Barrett's Esophageal Cells Induces Cancer‐Associated Changes

xlomafota13 shared this article with you from Inoreader Pepsinogen/Proton Pump Co‐Expression in Barrett's Esophageal Cells Induces Cancer‐Associated Changes Via The Laryngoscope Educational Objective At the conclusion of this presentation, participants should better understand the carcinogenic potential of pepsin and proton pump expression in Barrett's esophagus. Objective Barrett's esophagus (BE) is a well-known risk factor for esophageal adenocarcinoma (EAC). Gastric H+/K+ ATPase proton pump and pepsin expression has been demonstrated in some cases of BE; however, the contribution of local pepsin and proton pump expression to carcinogenesis is unknown. In this study, RNA sequencing was used to examine global transcriptomic changes in a BE cell line ectopically expressing pepsinogen and/or gastric H+/K+ ATPase proton pumps. Study Design In vitro translational. Methods BAR-T, a human BE cell line devoid of expression of pepsinogen or proton pumps, was transduced by lentivirus-encoding pepsinogen (PGA5) and/or gastric proton pump subunits (ATP4A, ATP4B). Changes relative to the parental line were assessed by RNA sequencing. Results Top canonical pathways associated with protein-coding genes differentially expressed in pepsinogen and/or proton pump expressing BAR-T cells included those involved in the tumor microenvironment and epithelial–mesenchymal transition. Top upstream regulators of coding transcripts included TGFB1 and ERBB2, which are associated with the pathogenesis and prognosis of BE and EAC. Top upstream regulators of noncoding transcripts included p300-CBP, I-BET-151, and CD93, which have previously described associations with EAC or carcinogenesis. The top associated disease of both coding and noncoding transcripts was cancer. Conclusions These data support the carcinogenic potential of pepsin and proton pump expression in BE and reveal molecular pathways affected by their expression. Further study is warranted to investigate the role of these pathways in carcinogenesis associated with BE. Level of Evidence N/A Laryngoscope, 2022 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

ROBOTIC REPAIR OF ATRIAL SEPTAL DEFECT WITH PARTIAL PULMONARY VENOUS RETURN ANOMALY: OUR 5‐YEAR EXPERIENCE

xlomafota13 shared this article with you from Inoreader ROBOTIC REPAIR OF ATRIAL SEPTAL DEFECT WITH PARTIAL PULMONARY VENOUS RETURN ANOMALY: OUR 5‐YEAR EXPERIENCE Via The International Journal of Medical Robotics and Computer Assisted Surgery ABSTRACT Introduction Partial pulmonary venous return anomalies (PPVRA) were not considered as a good candidate for robotic surgery in early time of robotic cardiac surgery. In this study, we present our experience in patients undergoing robotic atrial septal defect (ASD) and PPVRA surgery. Methods Between November 2014-January 2020, data of 21 patients underwent robotic ASD with PPVRA was collected. Inclusion criterion was presence of right-sided PPVRA with ASD. All operations were performed robotically. Results The mean age of patients was 26.7±10.3 years. 17 patients (81 %) had superior-caval ASD with supracardiac PPVRA and double-patch technique was used. Four patients had inferior-caval ASD with intracardiac PPVRA and single-patch technique was preferred. Cross-clamp time and cardiopulmonary bypass time were 92.8±29.6 and 127.8±38.1, respectively. There was no mortality. One patient had atrioventricular-block and required pacemaker. Conclusion Robotic repair of ASD with PPVRA is feasible and effective method as an alternative to conventional surgery. This article is protected by copyright. All rights reserved. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

The outcome of tympanic membrane grafting medial or lateral to malleus handle in type I underlay tympanoplasty

xlomafota13 shared this article with you from Inoreader The outcome of tympanic membrane grafting medial or lateral to malleus handle in type I underlay tympanoplasty Via Indian Journal of Otology Namrata Dwivedi, Shalini Jain, Ashok Kumar Indian Journal of Otology 2021 27(4):180-184 Aims: This study aims to study and compare the outcome of tympanic membrane grafting medial or lateral to handle of malleus in terms of graft uptake and closure of air bone gap. Materials and Methods: This prospective randomized study included 60 patients who presented in the ENT Outpatient Department of ABVIMS and Dr. Ram Manohar Lohia hospital with Inactive chronic Otitis media (COM) (tubo tympanic disease). Patients were randomly allocated to two groups of 30 patients each. Group A contained patients with graft placed medial to handle of malleus and Group B contained patients with graft placed lateral to handle of malleus. Patients were followed up at the 4th, 6th, and 12th week postoperatively for graft uptake and hearing gain were assessed. Results: There was significant mean hearing gain postoperatively compared to preoperative in pure tone audiometry, however, there is no significant difference in short-term outcomes of both the grafting techniques for graft uptake and audio m etric results. Conclusion: Both medial and lateral placement of grafting material to malleus handle into type I underlay tympanoplasty are good methods for graft uptake and audiological outcome for inactive COM (Tubo tympanic disease). View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Otogenic Cerebral Venous Sinus Thrombosis: A Case Report and Literature Review

xlomafota13 shared this article with you from Inoreader Otogenic Cerebral Venous Sinus Thrombosis: A Case Report and Literature Review Via Indian Journal of Otology Mostafa Meshref, Ahmed Taher Masoud, Amira Yasmine Benmelouka Indian Journal of Otology 2021 27(4):238-240 Cerebral venous sinus occlusion is an exceptional presentation in the emergency department. Otitis and mastoiditis are rarely associated with cerebral venous sinus occlusion. It is considered as a redoubtable complication with potentially severe outcomes. Symptoms that may indicate this thrombosis are variable and include progressive headache, intracranial hypertension, seizures, focal neurological deficits, and ophthalmologic symptoms. In this article, we report a case of a young female patient presenting with otitis and mastoiditis complicated with intracranial thrombosis. She was successfully treated with antibiotics, anticoagulants, and analgesics. Furthermore, we provide a brief review on cerebral venous sinus thrombosis with a focus on its risk factors and its management. Finally, we emphasize the need for better awareness about this potential intracranial complication. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

The Endonasal Endoscopic Approach to Different Sinonasal Fungal Balls

xlomafota13 shared this article with you from Inoreader The Endonasal Endoscopic Approach to Different Sinonasal Fungal Balls Via International Journal of Otolaryngology Background. Fungal ball sinusitis is a sinonasal fungus ball that usually affects immunocompetent adults with female predominance. The most affected sinus is the maxillary sinus. Aspergillus species is the most typically found fungus. Computed tomography (CT) scan is the gold standard tool in order to diagnose fungal ball sinusitis. The ultimate method for a fungal ball is functional endoscopic sinus surgery (FESS), which has a high success rate and a low morbidity rate. Objective. This study aims to demonstrate the various clinical presentations of fungal ball sinusitis including isolated maxillary sinus, sphenoid sinus, simultaneous occurrence of maxillary and sphenoid fungal ball, and post endonasal endoscopic pituitary surgery fungal ball with various age groups. Also, this study aims t o emphasize the importance of early diagnosis and treatment in such cases. Patients and Methods. A retrospective study that was carried in the otorhinolaryngology department of two hospitals: King Fahad Specialist Hospital and Qatif Central Hospital, Eastern Region, Saudi Arabia. The study was conducted on a total of 16 patients who were diagnosed with paranasal sinuses fungal ball in an 11-year period from January 2008 and November 2019. Results. Out of 16 patients with paranasal sinuses fungal ball, 11 cases were female and 5 males, with age ranging between 16 and 46 years. Results showed eight isolated sphenoid (50%), six isolated maxillary fungal ball (38%), one simultaneous occurrence of the sphenoid and maxillary fungal ball (6%), and one post endonasal endoscopic pituitary surgery for pituitary adenoma (6%). CT scan was performed for all 16 cases which is the standard tool for the diagnosis of the fungal ball. Conclusion. Fungal ball may present with variety of symptoms but m ost commonly with postnasal discharge (PND), headache, and facial pain. CT sinuses is the diagnostic radiological modality to confirm the diagnosis. The FESS functional endoscopic sinus surgery is the gold safe approach for patients with fungal ball to manage their symptoms, confirm the diagnosis, and removal of disease with no morbidities. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Injection laryngoplasty during transoral laser microsurgery for early glottic cancer: a randomized controlled trial

xlomafota13 shared this article with you from Inoreader Injection laryngoplasty during transoral laser microsurgery for early glottic cancer: a randomized controlled trial Via Journal of Otolaryngology - Head & Neck Surgery Transoral laser microsurgery is widely used for treating T1/T2 glottic cancers. Hyaluronic acid (HA) is commonly used in vocal cord augmentation. We investigated the impact of intra-operative injection laryngo... View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Evolution of midface microvascular reconstruction: three decades of experience from a single institution

xlomafota13 shared this article with you from Inoreader Evolution of midface microvascular reconstruction: three decades of experience from a single institution Via http://link.springer.com/journal/405,European archives of oto-rhino-laryngology,The European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino Eur Arch Otorhinolaryngol. 2022 Mar 22. doi: 10.1007/s00405-022-07321-x. Online ahead of print. ABSTRACT PURPOSE: Midface reconstruction poses a complex set of challenges for reconstructive surgeons. The optimal midface reconstruction must possess a durable underlying bone construct capable of integrating dental implants. Facial contour is restored by the overlying microvascular soft tissue reconstruction with reestablishment of the oral cavity. A plethora of microvascular flaps used in clinical practice have been described including those harvested from the iliac crest, scapula, fibula, forearm and back (latissimus dorsi). The objective was to share our experiences with each of these treatment options that have continued to evolve over time for the benefit of patients. METHODS: Our institution has over three decades of experience in reconstructing complex midface defects and this article summarizes midface reconstruction from an evolutionary perspective (for type II, III and IV defect; Browns classification, Supplementary Table I). We broadly divide this into (i) flaps supplied by the subscapular system (ii) autologous reconstruction with titanium mesh and (iii) fibula microvascular flaps using 3D planning. RESULTS: The advantages and disadvantages for each approach are discussed (Supplementary Table II). CONCLUSION: In the future, it is expected that 3D planning coupled with rapid prototyping, intraoperative navigation and CT imaging will become standard procedural practice. PMID:35316380 | DOI:10.1007/s00405-022-07321-x View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.