Bioelectrical impedance analysis for perioperative water management in adult cardiovascular valve disease surgery

xlomafota.13 shared this article with you from Inoreader Bioelectrical impedance analysis for perioperative water management in adult cardiovascular valve disease surgery Via Latest Results for Surgery Today Abstract Purpose Bioelectrical impedance analysis (BIA) has been used recently to measure the body water of patients with acute heart failure. We used BIA in this study to better understand, and possibly identify a predictive marker for, perioperative water behavior in cardiac surgery patients. Methods We measured body water and studied its behavior in 44 patients undergoing surgery for cardiac valvular disease at our hospital. Measurements included the levels of extracellular water (ECW), intracellular water (ICW), and total body water, the edema index (EI), and the ratio of ECW to total body water. The first measured EI was defined as the "preoperative EI" and the maximum as the "peak EI". Results A negative correlation was found between the preoperative EI and the preoperative estimated glomerular filtration rate (eGFR) (R = 0.644, p < 0.001). Positive correlations were found between the peak EI and the ICU stay (R = 0.625, p < 0.001), the peak EI and the ventilation time (R = 0.366, p < 0.01), and the preoperative EI and the ICU stay (R = 0.464, p = 0.026). Conclusion The EI is possibly a predictive marker for perioperative water management in cardiac surgery. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Use of an Alternative Pathway for Isoleucine Synthesis in Threonine-Producing Strains of Escherichia coli

xlomafota.13 shared this article with you from Inoreader Use of an Alternative Pathway for Isoleucine Synthesis in Threonine-Producing Strains of Escherichia coli Via Latest Results for Applied Biochemistry and Microbiology Abstract An E. coli strain in which all known pathways of threonine catabolism were inactivated (Δtdh, ΔltaE, ΔilvA, ΔtdcB, ΔyiaY) has been constructed. It was shown that the expression of heterologous citramalate synthase from Leptospira interrogans in an E. coli strain carrying the ΔilvA deletion can serve as an alternative pathway for isoleucine synthesis. It was observed that cimA overexpression has a negative effect on threonine production. We developed a system for regulated gene expression based on the inducible promoter PLtetO and TetR, a repressor of the tetracycline operon. The threonine-producing strain B-1201, in which the cimA gene is expressed under the control of the regulated promoter, was constructed. When the B-1201 strain was cultivated in a fermenter, a correlation was established between the threonine productivity and the expression level of th e cimA gene, and the optimal inductor content for the maximum threonine accumulation was also determined. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Assessment of the Biotechnological Potential of Cyanobacterial and Microalgal Strains from IPPAS Culture Collection

xlomafota.13 shared this article with you from Inoreader Assessment of the Biotechnological Potential of Cyanobacterial and Microalgal Strains from IPPAS Culture Collection Via Latest Results for Applied Biochemistry and Microbiology Abstract A search for strains capable of the simultaneous production of high amounts of several biologically valuable compounds and/or high biomass productivity has been carried out. The growth characteristics and biochemical composition of 12 microalgal and cyanobacterial strains from the IPPAS Collection were studied at the exponential and stationary growth phases. All of the strains had high growth rates (a doubling time of 6–22 h). The strains Cyanobacterium sp. IPPAS B-1200, Chlorella sp. IPPAS C-1210, Nannochloris sp. IPPAS C-1509, Cyanidium caldarium IPPAS P-510, and Vischeria sp. IPPAS H-242 demonstrated the highest biotechnological potential and can be used for the production of various types of biofuel, pigments, and feed and food additives, including those with a high content of eicosapentaenoic acid (20 : 5 Δ5, 8,11, 14, 17). View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

New Recombinant Phytase from Kosakonia sacchari : Characteristics and Biotechnological Potential

xlomafota.13 shared this article with you from Inoreader New Recombinant Phytase from Kosakonia sacchari : Characteristics and Biotechnological Potential Via Latest Results for Applied Biochemistry and Microbiology Abstract A DNA sequence from Kosakonia sacchari that, according to automated computer analysis, is believed to correspond to the gene for histidine-acid phytase has been selected from the GenBank database. The sequence was optimized for codon composition, synthesized, cloned, and expressed in Pichia pastoris. The main characteristics of the purified recombinant enzyme were determined. It was established that a pH of 4.5 and a temperature of 50°C are optimal for phytase functioning. The specific activity, Michaelis constant (Km), and maximum reaction rate (Vmax) with phytate as a substrate were 1470 U/mg, 193 μM and 2167 μmol/(min mg), respectively. It was shown that the enzyme was characterized by a wide range of working pH levels. Therefore, due to its properties, this new recombinant phytase can be considered a high-potential enzyme for agrobiotechnology. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Obtainment and Characteristics of Phytoecdysteroid-Producing Аjuga turkestanica (Rgl.) Plant Cell Cultures

xlomafota.13 shared this article with you from Inoreader Obtainment and Characteristics of Phytoecdysteroid-Producing Аjuga turkestanica (Rgl.) Plant Cell Cultures Via Latest Results for Applied Biochemistry and Microbiology Abstract Ajuga turkestanica (Rgl.) Briq. (Lamiaceae) callus cultures were obtained from a wild plant, and the morphogenesis processes in these cultures were studied. It was possible to obtain gemmogenesis or rhizogenesis in the first six to eight growth cycles, depending on the hormonal composition of the medium; the capacity for morphogenesis was lost after 10–15 growth cycles. Suspension cell cultures were initiated from some calluses with strong growth. As a result, about 100 calluses and suspension plant cell lines of A. turkestanica were obtained; a number of them were characterized in terms of growth rate and were analyzed via HPLC-MS for the presence of phytoecdysteroids. 20-Hydroxyecdysone and turkesterone were found in most of the studied lines; the content of the first compound in the most productive lines was 30–50 times higher than in the second (2.0–2.5 mg/g versus 0.04– 0.05 mg/g dry weight, respectively). An increase i n the phytoecdysteroid content in plant cells cultured in vitro was observed by the end of the growing cycle. However, phytoecdysteroids were not found in many of the obtained plant cell lines. Further research is needed to clarify the causes of the presence or absence of phytoecdysteroids in A. turkestanica plant cell cultures. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Effect of Virus-Inactivating Agents on the Immunogenicity of Hantavirus Vaccines against Hemorrhagic Fever with Renal Syndrome

xlomafota.13 shared this article with you from Inoreader Effect of Virus-Inactivating Agents on the Immunogenicity of Hantavirus Vaccines against Hemorrhagic Fever with Renal Syndrome Via Latest Results for Applied Biochemistry and Microbiology Abstract Hemorrhagic fever with renal syndrome (HFRS) is an acute zoonotic disease caused by the orthohantaviruses Puumala, Dobrava-Belgrad (four genotypes), Hantaan (genotype Amur), and Seoul. In the Russian Federation, HFRS occupies the leading place among all natural focal human diseases. Formaldehyde, β-propiolactone, and ultraviolet radiation were tested to select the optimal method for viral inactivation during the development of a whole-virion vaccine against HFRS. The specific activity and the immunogenicity of the vaccine were determined, respectively, by the number of copies of viral RNA per 1 mL and by the neutralizing antibodies in the blood sera of BALB/c mice in response to immunization. Analysis of the immunogenicity of vaccines inactivated with formaldehyde, β-propiolactone, or UV radiation did not find significant differences in the level of induced neutralizing antibodies. At the same time, β-propiolactone has obvious technological advantages as c ompared to formaldehyde and UV radiation: the time of viral inactivation is reduced by dozens of times; neutralization of the inactivator and control of its content in the final vaccine are not required; and the amount of inappropriate protein in the vaccine is decreased due to the decrease in protein aggregation. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Prospects for the Use of Methylotrophic Yeast in the Creation of Industrial Producers of Feed Enzymes

xlomafota.13 shared this article with you from Inoreader Prospects for the Use of Methylotrophic Yeast in the Creation of Industrial Producers of Feed Enzymes Via Latest Results for Applied Biochemistry and Microbiology Abstract In recent years, mycelial fungi have faced competition from recombinant yeast as producers of feed enzymes. An intensive study on genetic diversity identified the yeast genes encoding feed enzymes, the specific activity of which is much higher than that in mycelial fungi. In addition, these genes were expressed in yeast much more efficiently than in mycelial fungi. The use of yeast recombinant producers allowed the expansion of the production of a line of industrial enzymes with a significant reduction in their cost. The advantages of yeast producers of recombinant enzymes include the ability to obtain monoenzymes, which are part of various enzyme complexes used for different purposes. Pichia pastoris methylotrophic yeast is the most attractive subject for the creation of recombinant protein-producing strains. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.