Head-to-head comparison of two rapid high-throughput automated electrochemiluminescence immunoassays targeting total antibodies to the SARS-CoV-2 nucleoprotein and spike protein receptor binding domain

alkiviadis.1961 shared this article with you from Inoreader Head-to-head comparison of two rapid high-throughput automated electrochemiluminescence immunoassays targeting total antibodies to the SARS-CoV-2 nucleoprotein and spike protein receptor binding domain Via ScienceDirect Publication: Journal of Clinical Virology Publication date: Available online 5 March 2021 Source: Journal of Clinical Virology Author(s): Mario Poljak, Anja Oštrbenk Valenčak, Tina Štamol, Katja Seme View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Midazolam for sedation before procedures in adults and children: a systematic review update

alkiviadis.1961 shared this article with you from Inoreader Midazolam for sedation before procedures in adults and children: a systematic review update Via Systematic Reviews - Latest Articles Midazolam is used for sedation before diagnostic and therapeutic medical procedures by several routes including oral, intravenous, intranasal and intramuscular. This is an update of a Cochrane review published... View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

COVID-19 related increase in childhood tics and tic-like attacks

alkiviadis.1961 shared this article with you from Inoreader COVID-19 related increase in childhood tics and tic-like attacks Via ADC Online First Explosion of tics Since the onset of the COVID-19 pandemic, paediatricians and child mental health practitioners have noticed an increase in tic symptoms in some children and adolescents already diagnosed with tic disorders.1 Interestingly, clinicians have also seen a marked increase in presentations of sudden and new onset of severe tics and 'tic-like' attacks. There is an urgent need to collate systematic data on this group as this is a rare and unusual subtype of tics and Tourette syndrome , differing in age and type of onset and expected patterns of tics. Typically, childhood tics start around 5–7 years and show a waxing and waning course of predominantly motor tics, more commonly affecting boys in a ratio of 4:1. The new surge of referrals consists of adolescent girls with sudden onset of motor and phonic tics of a complex and bizarre nature. In London, UK specialist tic clinics at each... View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Longitudinal assessment of anti-SARS-CoV-2 immune responses for six months based on the clinical severity of COVID-19

alkiviadis.1961 shared this article with you from Inoreader Longitudinal assessment of anti-SARS-CoV-2 immune responses for six months based on the clinical severity of COVID-19 Via Journal of Infectious Diseases - Advance Access Abstract There is insufficient data on the longevity of immunity acquired following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We aimed to evaluate the duration of SARS-CoV-2-specific humoral and cellular immunity according to the clinical severity of coronavirus disease 2019 (COVID-19). The study population comprised asymptomatic (n=14), symptomatic/non-pneumonic (n=42), and pneumonic (n=41) patients. The anti-SARS-CoV-2 IgG and neutralizing antibody (NAb) titers lasted until six mont hs after diagnosis, with positivity rates of 66.7% and 86.9%, respectively. Older age, prolonged viral shedding and accompanying pneumonia were more frequently found in patients with sustained humoral immunity. SARS-CoV-2 specific T-cell response was strongly observed in pneumonic patients and prominent in individuals with sustained humoral immunity. In conclusion, most (> 85%) patients carries NAb until six months after diagnosis of SARS-CoV-2 infection, providing insights for establishing vaccination strategies against COVID-19. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Sex differences in the evolution of neutralizing antibodies to SARS-CoV-2

alkiviadis.1961 shared this article with you from Inoreader Sex differences in the evolution of neutralizing antibodies to SARS-CoV-2 Via Journal of Infectious Diseases - Advance Access Abstract We measured Anti-Spike (S), Nucleoprotein (N) and neutralizing antibodies (NAbs) in sera from 308 RT-qPCR + healthcare workers with mild disease, collected at two time-points up to 6 months after symptom onset. At Month 1 (M1), anti-S and N antibody levels were higher in males > 50 years or with a body mass index (BMI) > 25. At M3-6, anti-S and anti-N antibodies were detected in 99% and 59% of individuals, respectively. Anti-S antibodies and NAbs declined faster in males than in females, independently of age and BMI, suggesting an association of sex with evolution of the humoral response. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Shedding of culturable virus, seroconversion, and 6-month follow-up antibody responses in the first 14 confirmed cases of COVID-19 in the United States

alkiviadis.1961 shared this article with you from Inoreader Shedding of culturable virus, seroconversion, and 6-month follow-up antibody responses in the first 14 confirmed cases of COVID-19 in the United States Via Journal of Infectious Diseases - Advance Access Abstract We aimed to characterize presence of culturable virus in clinical specimens during acute illness, and antibody kinetics up to six months post-onset, among 14 early US COVID-19 patients. We isolated viable SARS-CoV-2 from rRT-PCR-positive respiratory specimens collected during days 0-8 post-onset, but not after. All 13 patients with two or more serum specimens developed anti-spike antibodies; 12 developed detectable neutralizing antibodies. We did not isolate virus after detection of neutralizing antibodie s. Eight participants provided serum at six months post-onset; all retained detectable anti-spike IgG, and half had detectable neutralizing antibodies. Two participants reported not feeling fully recovered at six months. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Failure of nonoperative management in patients with acute diverticulitis complicated by abscess: a systematic review

alkiviadis.1961 shared this article with you from Inoreader Failure of nonoperative management in patients with acute diverticulitis complicated by abscess: a systematic review Via Latest Results for International Journal of Colorectal Disease Abstract Background The aim of this study was to assess failure rates following nonoperative management of acute diverticulitis complicated by abscess and trends thereof. Method Pubmed, MEDLINE, EMBASE, CINAHL, Cochrane Library, and Web of Science were systematically searched. Nonoperative management was defined as a combination of nil per os, IV fluids, IV antibiotics, CT scan–guided percutaneous drainage, and total parenteral nutrition. The primary endpoint was failure of nonoperative management defined as persistent or worsening abscess and/or sepsis, development of new complications, such as peritonitis, ileus, or colocutaneous fistula, and urgent surgery within 30–90 days of index admission. Data were stratified by three arbitrary time intervals: 1986–2000, 2000–2010, and after 2010. The primary outcome was calculated for those groups and compared. Results Thirty-eight of forty-four eligible studies published between 1986 and 2019 were included in the quantitative synthesis of data (n = 2598). The pooled rate of failed nonoperative management was 16.4% (12.6%, 20.2%) at 90 days. In studies published in 2000–2010 (n = 405), the pooled failure rate was 18.6% (10.5%, 26.7%). After 2000 (n = 2140), the pooled failure rate was 15.3% (10.7%, 20%). The difference was not statistically significant (p = 0.725). After controlling for heterogeneity in the definition of failure of nonoperative management, subgroup analysis yielded the pooled rate of failure of 21.8% (16.1%, 27.4%). Conclusion This meta-analysis found that failure rates following nonoperative management of acute diverticulitis complicated by abscess did not significantly decrease over the past three decades. The general quality of published data and the level and certainty of evidence produced were low. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.