Columellar strut grafts versus septal extension grafts during rhinoplasty for airway function, patient satisfaction and tip support

xlomafota13 shared this article with you from Inoreader Columellar strut grafts versus septal extension grafts during rhinoplasty for airway function, patient satisfaction and tip support Via Journal of plastic, reconstructive & aesthetic surgery : JPRAS J Plast Reconstr Aesthet Surg. 2022 Feb 24:S1748-6815(22)00095-X. doi: 10.1016/j.bjps.2022.02.017. Online ahead of print. ABSTRACT IMPORTANCE: Different techniques exist to provide tip support in rhinoplasty. There is little evidence to provide a consensus on the most effective choice. OBJECTIVE: Evaluating columellar strut graft (CSG) and septal extension grafts (SEG) for their influence on airway function, patient satisfaction and tip support. DESIGN, SETTINGS AND PARTICIPANTS: A retrospective cohort study was undertaken on 165 adult patients who underwent open rhinoplasty with either a CSG or SEG, from February 2012 to August 2019 in a single tertiary facial-plastic practice in Sydney, Australia. Operations were for both cosmetic and functional indications, and both primary and revision cases were assessed. Airway testing and patient-reported outcomes (PROMs) were performed preoperatively and at least 6 months following the procedure. Photographic tip analysis was taken from approximately 4 and 12-month postoperative photographs. MAIN OUTCOMES AND MEASURES: Nasal peak inspiratory flow (NPIF) and total nasal airway resistance (NAR) were the primary airway functional outcomes. The primary PROMs analysed were a visual analogue scale (VAS) for nasal obstruction and 13-point Likert scale for global cosmesis, the Nose Outcome Symptom Evaluation (NOSE), and the nasal obstruction score. Tip support was determined by the nasolabial angle (NLA) and Simon's ratio as assessed by Rhinobase developed by Apaydin et al. on lateral Frankfort plane photographs. Data normalised as an improvement over preoperative baseline, accounting for individual variability. RESULTS: A total of 165 patients was assessed (35.2 ± 12.9 yrs, 72% female), 100 (61%) of which received SEG. There were similar nasal airway assessments between CSG and SEG groups, with ΔNPIF (20.0 ± 42.1 L/min v 19.9 ± 44.9 L/min, p = 0.983) and Δ "obstructed" NAR (-1.13 ± 1.90 v -1.02 ± 4.33 Pa/cm3/s, p = 0.849). Amongst PROMs, a greater cosmetic outcome was seen in the SEG group (7.20 ± 2.97 v 5.69 ± 3.45, p < 0.01) with all other assessments similar between CSG and SEG techniques. Photographic analysis of tip projection showed reduced NLA distortion in the SEG. CONCLUSION AND RELEVANCE: While greater patient-perceived cosmesis was seen in patients with a SEG, there were similar airflow and patient-reported nasal function between groups. Photographic analysis of tip projection showed SEG patients additionally benefited from less NLA distortion and greater tip maintenance. PMID:35337759 | DOI:10.1016/j.bjps.2022.02.017 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

H2O2 Concentration in Exhaled Breath Condensate Increases After Phonotrauma: A Promise of Noninvasive Monitoring?

xlomafota13 shared this article with you from Inoreader H2O2 Concentration in Exhaled Breath Condensate Increases After Phonotrauma: A Promise of Noninvasive Monitoring? Via Journal of Voice The present study was designed to observe the concentration of hydrogen peroxide (H2O2) in exhaled breath condensate (EBC) after induced phonotrauma. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

The application of polyethylene glycol‐coated collagen patches in nasal surgery

xlomafota13 shared this article with you from Inoreader The application of polyethylene glycol‐coated collagen patches in nasal surgery Via Clinical Otolaryngology Abstract Nasal packing is a significant factor of postoperative morbidity that affects quality of recovery following nasal surgery. The polyethylene glycol-coated (PEG) collagen patch (Hemopatch®) is a synthetic hemostatic agent with a dual mechanism of action, but whether it can be applied in nasal surgery remains unexplored. The postoperative symptoms of thick nasal discharge, loss of smell, headache, postnasal discharge, ear fullness and lack of good night's sleep in patients with Hemopatch are minimal after surgery. The total nasal resistance at postoperative day 1 (0.224 Pa/cm3) is lower than the preoperative total nasal resistance (0.412 Pa/cm3), indicating that the nasal air flow increases after surgery in patients using Hemopatch at postoperative day 1. This is the first report to demonstrate that Hemopatch is an alternative nasal packing to reduce the postoperative morbidity after nasal surgery. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

MicroRNA-302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1

xlomafota13 shared this article with you from Inoreader MicroRNA-302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1 Via Experimental and therapeutic medicine Exp Ther Med. 2022 Apr;23(4):298. doi: 10.3892/etm.2022.11227. Epub 2022 Feb 21. ABSTRACT [This retracts the article DOI: 10.3892/etm.2018.6223.]. PMID:35317449 | PMC:PMC8908467 | DOI:10.3892/etm.2022.11227 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Micropulse vs. continuous wave transscleral cyclophotocoagulation in neovascular glaucoma

xlomafota13 shared this article with you from Inoreader Micropulse vs. continuous wave transscleral cyclophotocoagulation in neovascular glaucoma Via Experimental and therapeutic medicine Exp Ther Med. 2022 Apr;23(4):278. doi: 10.3892/etm.2022.11207. Epub 2022 Feb 11. ABSTRACT Neovascular glaucoma (NVG) is a refractory form of glaucoma, associated with important morbidity, for which no consensus exists regarding the optimal choice of therapy. The primary aim of our study was to compare the performances of micropulse transscleral cyclophotocoagulation (MP-TSCPC) and continuous wave transscleral cyclophotocoagulation (CW-TSCPC) in the treatment of neovascular glaucoma (NVG). A total of 24 eyes for MP-TSCPC and 22 eyes for CW-TSCPC, all with NVG were included. The procedures were performed using either the Iridex Cyclo G6 (IRIDEX Laser System), the MP3, or the G-Probe devices. Intraocular pressure (IOP), visual acuity (VA), the mean number of antiglaucoma medications, and postoperative complications were monitored. The minimum follow-up was 12 months. The success rate at 12 months was 54.5% in the CW-TSCPC group and 33.3% in the MP-TSCPC group. The mean IOP at baseline was 35.82 mm Hg for CW-TSCPC and 34.71 mm Hg for MP-TSCPC. The change from baseline in IOP at 12 months was 11.95 mm Hg in the CW-TSCPC group and -8.04 mm Hg in the MP-TSCPC group. There was a significant difference in the occurrence of serious complications (worsening of VA, hypotony, and phthisis bulbi) between the two methods, with CW-TSCPC associated with more important adverse effects (P=0.045). There was a decrease in the number of topical antiglaucoma medications in both groups: in the MP-TSCPC group from a mean number of 2.6 at baseline, to 1.7 at 3 months, followed by a slight increase to 2.1 at 12 months and in the CW-TSCPC group from 2.8 at baseline, to 1.4 at 3 months and 1.9 at 12 months. Our study concluded that both MP-TSCPC and CW-TSCPC could manage NVG, but, while CW-TSCPC revealed higher IOP control in the long term (which did not reach statistical significance), it also had a significantly lower safety profile. PMID:35317447 | PMC:PMC8908348 | DOI:10.3892/etm.2022.11207 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Circular RNA hsa_circ_0011946 promotes the malignant process of salivary adenoid cystic carcinoma by downregulating miR-1205 expression

xlomafota13 shared this article with you from Inoreader Circular RNA hsa_circ_0011946 promotes the malignant process of salivary adenoid cystic carcinoma by downregulating miR-1205 expression Via Experimental and therapeutic medicine Exp Ther Med. 2022 Apr;23(4):295. doi: 10.3892/etm.2022.11224. Epub 2022 Feb 18. ABSTRACT Circular RNA (circRNA/circ) hsa_circ_0011946 has been reported to serve an important role in a number of cancer types; however, to the best of our knowledge, its role in salivary adenoid cystic carcinoma (SACC) has not been reported. In the present study, the primary focus was the effects of hsa_circ_0011946 on the invasion, migration and epithelial-mesenchymal transformation (EMT) of SACC cells, and the specific mechanisms involved. The expression levels of hsa_circ_0011946 and microRNA (miR)-1205 in cancer tissues and paracancerous tissues of patients with SACC were analyzed using reverse transcription-quantitative (RT-q)PCR. The cell proliferation rate was determined using a Cell Counting Kit-8 assay. Wound healing assays were performed to analyze the cell migratory ability, while a transwell assay was used to measure the cell invasion ability. Western blotting was used to analyze the expression levels of EMT-related proteins. Cell transfection was used to knockdown hsa_circ_0011946 and knockdown or overexpress miR-1205. Subcellular localization assays for hsa_circ_0011946 were performed using RT-qPCR. A dual-luciferase reporter gene assay was used to verify the binding between hsa_circ_0011946 and miR-1205. The results of the present study revealed that the expression levels of hsa_circ_0011946 were significantly upregulated in cancer tissues from patients with SACC. The knockdown of hsa_circ_0011946 expression inhibited the proliferation, invasion and migration of SACC cells, thereby inhibiting the EMT process, which was achieved by downregulating miR-1205 expression. In conclusion, circRNA hsa_circ_0011946 was discovered to promote the malignant process of SACC by downregulating miR-1205 expression. PMID:35317442 | PMC:PMC8908474 | DOI:10.3892/etm.2022.11224 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Anagliptin promotes apoptosis in mouse colon carcinoma cells via MCT-4/lactate-mediated intracellular acidosis

xlomafota13 shared this article with you from Inoreader Anagliptin promotes apoptosis in mouse colon carcinoma cells via MCT-4/lactate-mediated intracellular acidosis Via Experimental and therapeutic medicine Exp Ther Med. 2022 Apr;23(4):282. doi: 10.3892/etm.2022.11211. Epub 2022 Feb 15. ABSTRACT Cancer cells frequently exhibit an acidic extracellular microenvironment, where inversion of the transmembrane pH gradient is associated with tumor proliferation and metastasis. To elucidate a new therapeutic target against cancer, the current study aimed to determine the mechanism by which the dipeptidyl peptidase-4 inhibitor anagliptin regulates the cellular pH gradient and concomitant extracellular acidosis during cancer progression. A total of 5x105 CT-26 cells (resuspended in phosphate buffer saline) were injected subcutaneously in the right flank of male BALB/c mice (weighing 25-28 g). The tumor samples were harvested, and lactate was detected using a lactate assay kit. Immunohistochemistry was used to detect the Ki67 and PCNA. MTT assay and flow cytometric were used to detect cell viability. Intracellular pH was detected by fluor escence pH indicator. The results revealed that anagliptin effectively reduced tumor growth, but did not affect the body weight of treated mice. Anagliptin reduced the accumulation of lactate in tumor sample. Treatment with anagliptin stimulated the apoptosis of CT-26 cells. And lactate excretion inhibition is accompanied by an increase in extracellular pH (pHe) after treatment with anagliptin. Furthermore, anagliptin induced intracellular acidification and reversed the low pHe gradient via monocarboxylate transporter-4 (MCT-4)-mediated lactate excretion. Additionally, anagliptin reversed the aberrant transmembrane extracellular/intracellular pH gradient by suppressing MCT-4-mediated lactate excretion, while also reducing mitochondrial membrane potential and inducing apoptosis. These data revealed a novel function of anagliptin in regulating lactate excretion from cancer cells, suggesting that anagliptin may be used as a potential treatment for cancer. PMID:35317435 | PMC:PMC8908463 | DOI:10.3892/etm.2022.11211 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.