Young Barley Indicates Antitumor Effects in Experimental Breast Cancer In Vivo and In Vitro.

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Young Barley Indicates Antitumor Effects in Experimental Breast Cancer In Vivo and In Vitro.

Nutr Cancer. 2016 Apr 4;:1-11

Authors: Kubatka P, Kello M, Kajo K, Kruzliak P, Výbohová D, Šmejkal K, Maršík P, Zulli A, Gönciová G, Mojžiš J, Kapinová A, Murin R, Péč M, Adamkov M, Przygodzki RM

Abstract
The effect of dietary administered young barley containing a mixture of phytochemicals to female rats for the prevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis was evaluated. After carcinogen administration (14 wk), mammary tumors were removed and prepared for histopathological and immunohistochemical analysis. Moreover, in vitro evaluation of possible mechanisms in MCF-7 breast cancer cell line was performed. Barley (0.3%) demonstrated mild antitumor effect in mammary carcinogenesis, yet 3% barley did not further improve this effect. Immunohistochemical analysis of rat tumor cells in treated groups showed significant increase in caspase-3 expression and significant reduction in Ki67 expression. In addition, 3% barley significantly decreased dityrosine levels versus control. Barley in higher dose significantly decreased serum low-density lipoprotein-cholesterol in rats. In vitro studies showed that barley significantly decreased survival of MCF-7 cells in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and significantly decreased 5-bromo-20-deoxyuridine incorporation versus control. Barley prevented cell cycle progression and extended incubation with barley showed significant increase in the percentage of annexin V/propidium iodide-positive MCF-7 cells. Our results propose an antitumor effect for the mixture of phytochemicals present in young barley in a breast cancer model.

PMID: 27042893 [PubMed - as supplied by publisher]

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