Established gastric cancer cell lines transplantable into C57BL/6 mice demonstrate FGFR4 promotion of tumor growth

Abstract

Previously no mouse gastric cancer cell lines have been available for transplantation into C57BL/6 mice. However, a gastric cancer model in immunocompetent mice would be useful for analyzing putative therapies.MNU was given in drinking water to C57BL/6 mice and p53 heterozygous knockout mice. Only one tumor from a p53 knockout mouse could be cultured and the cells are subcutaneously transplantable into a C57BL/6 mouse. We cultured this subcutaneous tumor, and sub-cloned it. The mRNA expression in the most aggressive YTN16 subline was compared to the less aggressive YTN2 subline by microarray analysis, and FGFR4 in YTN16 cells was knocked-out with a CRISPR/Cas9 system and inhibited by an FGFR4 selective inhibitor, BLU9931.

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