Noninvasive quantitation of rat cerebral blood flow using 99m Tc-HMPAO—assessment of input function with dynamic chest planar imaging

Abstract

Background

Cerebral blood flow (CBF) quantitation using technetium-99m hexamethylpropyleneamine oxime (^99mTc-HMPAO) generally requires assessment of input function by arterial blood sampling, which would be invasive for small animals. We therefore performed chest dynamic planar imaging, instead of arterial blood sampling, to estimate the input function and establish noninvasive quantitation method of rat CBF using the image-derived input function.

Results

Integrated radioactivity concentration in the heart-blood pool on planar images (AUC_Blood-planar) was identical to that in arterial blood samples (AUC_Blood-sampling). Radioactivity concentration in the brain determined by SPECT imaging (C_Brain-SPECT) was identical to that using brain sampling (C_Brain-sampling). Noninvasively calculated CBF obtained by dividing C_Brain-SPECT by AUC_Blood-planar was well correlated with conventionally estimated CBF obtained by dividing C_Brain-sampling by AUC_Blood-sampling.

Conclusion

Rat CBF could be noninvasively quantitated using ^99mTc-HMPAO chest dynamic planar imaging and head SPECT imaging without arterial blood sampling.

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