A Novel Reagent for Radioiodine Labeling of New Chemical Entities (NCEs) and Biomolecules

xlomafota13 shared this article with you from Inoreader A Novel Reagent for Radioiodine Labeling of New Chemical Entities (NCEs) and Biomolecules Via radioactive iodine Molecules. 2021 Jul 18;26(14):4344. doi: 10.3390/molecules26144344. ABSTRACT Radioiodine labeling of peptides and proteins is routinely performed by using various oxidizing agents such as Chloramine T, Iodobeads, and Iodogen reagent and radioactive iodide (I-), although some other oxidizing agents were also investigated. The main objective of the present study was to develop and test a novel reagent, inorganic monochloramine (NH2Cl), for radioiodine labeling o f new chemical entities and biomolecules which is cost-effective, easy to make and handle, and is selective to label amino acids, peptides, and proteins. The data presented in this report demonstrate that the yields of the non-radioactive iodine labeling reactions using monochloramine are >70% for an amino acid (tyrosine) and a cyclic peptide (cyclo Arg-Gly-Asp-d-Tyr-Lys, cRGDyK). No evidence of the formation of N-chloro derivatives in cRGDyK was observed, suggesting that the reagent is selective in iodinating the tyrosine residue in the biomolecules. The method was successfully translated into radioiodine labeling of amino acid, a peptide, and a protein, Bovine Serum Albumin (BSA). PMID:34299619 | DOI:10.3390/molecules26144344 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.