Fractionation spares mice from radiation-induced reductions in weight gain but does not prevent late oligodendrocyte lineage side effects

Publication date: Available online 12 May 2016
Source:International Journal of Radiation Oncology*Biology*Physics
Author(s): Sage Begolly, Peter G. Shrager, John A. Olschowka, Jacqueline P. Williams, M. Kerry O'Banion
PurposeTo determine the late effects of fractionated versus single dose cranial radiation on murine white matter.Methods and MaterialsMice were exposed to 0 Gy, 6x6 Gy, or 1x20 Gy cranial irradiation at 10-12 weeks of age. Endpoints were assessed through 18 months from exposure using immunohistochemistry, electron microscopy, and electrophysiology.ResultsWeight gain was temporarily reduced after irradiation; greater loss was seen after single versus fractionated doses. Oligodendrocyte progenitor cells were reduced early and late after both single and fractionated irradiation. Both protocols also increased myelin g-ratio, reduced the number of nodes of Ranvier, and promoted a shift in the proportion of small, unmyelinated versus large, myelinated axon fibers.ConclusionsFractionation does not adequately spare normal white matter from late radiation side effects.

Teaser

Although fractionation reduced cranial radiation-related effects on weight gain, patterns of early and late oligodendrocyte progenitor cell depletion and late white matter pathology and dysfunction were similar following both single and fractionated irradiation. This indicates that fractionation fails to reduce late white matter side effects of radiation in adult mice.


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