AbstractObjectivesTumour‐targeted gene therapy is a promising approach for effective control of gastric cancer cell proliferation. Our study aims to develop a cancer therapy which combines tumour‐targeting promoters with cytotoxins.MethodsThe expression of globotriaosylceramide (Gb3), which is a Shiga‐like toxin I (Stx1) receptor, was verified in gastric cancer compared with normal stomach tissues as assessed by flow cytometry and immunohistochemical analysis. We therefore constructed...
Sat Apr 06, 2019 19:10
AbstractObjectivesMesenchymal stem cells (MSCs) could regulate the function of various immune cells. It remains unclear whether MSCs additionally possess immunostimulatory properties. We investigated the impact of human MSCs on the responsiveness of primary natural killer (NK) cells in terms of induction of anti‐inflammatory purinergic signalling.Material and MethodsWe obtained human bone marrow mesenchymal stem cells (BMMSCs) and dental pulp stem cells (DPSCs). NK cells were isolated...
Fri Apr 05, 2019 19:09
AbstractObjectivesTo investigate the role of hypoxia in vasculogenic mimicry (VM) of salivary adenoid cystic carcinoma (SACC) and the underlying mechanism involved.Materials and methodsFirstly, wound healing, transwell invasion, immunofluorescence and tube formation assays were performed to measure the effect of hypoxia on migration, invasion, EMT and VM of SACC cells, respectively. Then, immunofluorescence and RT‐PCR were used to detect the effect of hypoxia on VE‐cadherin and...
Wed Apr 03, 2019 17:20
AbstractRecently, fibroblast growth factors are identified to play a vital role in the development and progression of human pancreatic cancer. FGF pathway is critical involved in numerous cellular processes through regulation of its downstream targets, including proliferation, apoptosis, migration, invasion, angiogenesis and metastasis. In this review article, we describe recent advances of FGFR signalling pathway in pancreatic carcinogenesis and progression. Moreover, we highlight...
Wed Apr 03, 2019 16:53
The molecular machinery of regulated cell deathThe molecular machinery of regulated cell death, Published online: 04 April 2019; doi:10.1038/s41422-019-0164-5The molecular machinery of regulated cell death
Thu Apr 04, 2019 03:00
Hierarchical activation of compartmentalized pools of AMPK depends on severity of nutrient or energy stressHierarchical activation of compartmentalized pools of AMPK depends on severity of nutrient or energy stress, Published online: 04 April 2019; doi:10.1038/s41422-019-0163-6Hierarchical activation of compartmentalized pools of AMPK depends on severity of nutrient or energy stress
Thu Apr 04, 2019 03:00
Cells, Vol. 8, Pages 324: The Importance of the Knee Joint Meniscal Fibrocartilages as Stabilizing Weight Bearing Structures Providing Global Protection to Human Knee-Joint Tissues Cells doi: 10.3390/cells8040324 Authors: Melrose The aim of this study was to review aspects of the pathobiology of the meniscus in health and disease and show how degeneration of the meniscus can contribute to deleterious changes in other knee joint components. The menisci, distinctive semilunar weight bearing...
Sat Apr 06, 2019 03:00
Cells, Vol. 8, Pages 322: CRISP-R/Cas9 Mediated Deletion of Copper Transport Genes CTR1 and DMT1 in NSCLC Cell Line H1299. Biological and Pharmacological Consequences Cells doi: 10.3390/cells8040322 Authors: Ekaterina Y. Ilyechova Elisa Bonaldi Iurii A. Orlov Ekaterina A. Skomorokhova Ludmila V. Puchkova Massimo Broggini Copper, the highly toxic micronutrient, plays two essential roles: it is a catalytic and structural cofactor for Cu-dependent enzymes, and it acts as...
Sat Apr 06, 2019 03:00
Cells, Vol. 8, Pages 323: The Potential Use of Metformin, Dipyridamole, N-Acetylcysteine and Statins as Adjunctive Therapy for Systemic Lupus Erythematosus Cells doi: 10.3390/cells8040323 Authors: Marcus Kai Xuan Tan Thurston Yan Jia Heng Anselm Mak Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune condition that can potentially affect every single organ during the course of the disease, leading to increased morbidity and mortality, and reduced health-related...
Sat Apr 06, 2019 03:00
Cells, Vol. 8, Pages 321: Updates on the CDK4/6 Inhibitory Strategy and Combinations in Breast Cancer Cells doi: 10.3390/cells8040321 Authors: Sobhani D'Angelo Pittacolo Roviello Miccoli Corona Bernocchi Generali Otto Breast Cancer (BC) is the second most common type of cancer worldwide and displays the highest cancer-related mortality among women worldwide. Targeted therapies have revolutionized the way BC has been treated in recent decades, improving...
Sat Apr 06, 2019 03:00
Cells, Vol. 8, Pages 320: Combined Inhibition of TGF-β Signaling and the PD-L1 Immune Checkpoint Is Differentially Effective in Tumor Models Cells doi: 10.3390/cells8040320 Authors: Sow Ren Camps Ossendorp ten Dijke Antibodies blocking the programmed death-ligand 1 (PD-L1) have shown impressive and durable responses in clinical studies. However, this type of immunotherapy is only effective in a subset of patients and not sufficient for rejection of all tumor types. In...
Fri Apr 05, 2019 03:00
Cells, Vol. 8, Pages 319: Apolipoprotein A-IV: A Multifunctional Protein Involved in Protection against Atherosclerosis and Diabetes Cells doi: 10.3390/cells8040319 Authors: Qu Ko Tso Bhargava Apolipoprotein A-IV (apoA-IV) is a lipid-binding protein, which is primarily synthesized in the small intestine, packaged into chylomicrons, and secreted into intestinal lymph during fat absorption. In the circulation, apoA-IV is present on chylomicron remnants, high-density lipoproteins,...
Fri Apr 05, 2019 03:00
Cells, Vol. 8, Pages 315: Cell-Free Protein Synthesis: Chassis toward the Minimal Cell Cells doi: 10.3390/cells8040315 Authors: Yue Zhu Kai The quest for a minimal cell not only sheds light on the fundamental principles of life but also brings great advances in related applied fields such as general biotechnology. Minimal cell projects came from the study of a plausible route to the origin of life. Later on, research extended and also referred to the construction of artificial...
Fri Apr 05, 2019 03:00
Cells, Vol. 8, Pages 317: Restoration of HCV-Specific Immune Responses with Antiviral Therapy: A Case for DAA Treatment in Acute HCV Infection Cells doi: 10.3390/cells8040317 Authors: Casey Feld MacParland Worldwide, 71 million individuals are chronically infected with Hepatitis C Virus (HCV). Chronic HCV infection can lead to potentially fatal outcomes including liver cirrhosis and hepatocellular carcinoma. HCV-specific immune responses play a major role in viral control and...
Fri Apr 05, 2019 03:00
Cells, Vol. 8, Pages 316: Structure and Dynamics of the EGF Receptor as Revealed by Experiments and Simulations and Its Relevance to Non-Small Cell Lung Cancer Cells doi: 10.3390/cells8040316 Authors: Martin-Fernandez Clarke Roberts Zanetti-Domingues Gervasio The epidermal growth factor receptor (EGFR) is historically the prototypical receptor tyrosine kinase, being the first cloned and the first where the importance of ligand-induced dimer activation was ascertained....
Fri Apr 05, 2019 03:00
Cells, Vol. 8, Pages 318: Transcriptomic Response to 1,25-Dihydroxyvitamin D in Human Fibroblasts with or without a Functional Vitamin D Receptor (VDR): Novel Target Genes and Insights into VDR Basal Transcriptional Activity Cells doi: 10.3390/cells8040318 Authors: Costa França Katayama Carneiro Martin Folgueira Latronico Ferraz-de-Souza The vitamin D receptor (VDR) mediates vitamin D actions beyond bone health. While VDR activation by 1,25-dihydroxyvitamin...
Fri Apr 05, 2019 03:00
Cells, Vol. 8, Pages 314: Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) Cells doi: 10.3390/cells8040314 Authors: Xiaoqing Li Ang Lu Wenjie Deng Li Su Jing Wang Mingyue Ding We report an imaging approach applying the atomic force acoustic microscopy (AFAM), which has unique potential for nondestructive imaging of cell internal structures. To obtain high spatial resolution images, we optimized the significant imaging parameters, including...
Fri Apr 05, 2019 03:00
Cells, Vol. 8, Pages 313: SVR12 Higher than 97% in GT3 Cirrhotic Patients with Evidence of Portal Hypertension Treated with SOF/VEL without Ribavirin: A Nation-Wide Cohort Study Cells doi: 10.3390/cells8040313 Authors: Mangia Cenderello Copetti Verucchi Piazzolla Lorusso Santoro Squillante Orlandini Minisini Ciancio In clinical trials, a sofosbuvir/velpatasvir (SOF/VEL) pangenotypic single-tablet regimen was associated with high sustained virological...
Thu Apr 04, 2019 03:00
Publication date: Available online 6 April 2019Source: Cellular SignallingAuthor(s): Zhen Yuan, Liang Zhong, Dongdong Liu, Juanjuan Yao, Junmei Liu, Pengqiang Zhong, Shifei Yao, Yi Zhao, Lianwen Li, Min Chen, Lu Liu, Beizhong LiuAbstractMicroRNAs have been shown to be involved in various cell processes, including proliferation, apoptosis and differentiation. However, little is known about their function in granulopoiesis. In the present study, overexpression and knockdown experiments revealed that...
Sun Apr 07, 2019 19:43
Publication date: Available online 5 April 2019Source: Cellular SignallingAuthor(s): Xiaoqin La, Lichao Zhang, Yufei Yang, Hanqing Li, Guisheng Song, Zhuoyu LiAbstractGlucose-regulated protein 78 (GRP78), an important molecular chaperone in the endoplasmic reticulum, is often over-expressed in the central region of advanced tumor and acts as a promoter of tumor progression. As main immune cells in the tumor microenvironment, infiltration of abundant macrophages into advanced tumor further facilitates...
Sat Apr 06, 2019 17:25
Publication date: Available online 3 April 2019Source: Cellular SignallingAuthor(s): Xin-Meng Wang, Qi-Yang Li, Li-Li Ren, Yi-Meng Liu, Tian-Shi Wang, Tian-Chi Mu, Shuai Fu, Chao Liu, Jian-Ying XiaoAbstractMicrospherule protein 1(MCRS1) is known to be an oncogene in several tumors. However, recent studies have shown that MCRS1 inhibits lymphatic metastasis in gastric cancer (GC) patients by inhibiting telomerase activity. Protein kinase, membrane associated tyrosine/threonine 1(Pkmyt1), a member...
Thu Apr 04, 2019 13:50
miR‐675 targets transforming growth factor beta receptor 1 (TGFβR1) to attenuate transforming growth factor beta 1 (TGFβ1)‐induced mouse cardiac fibroblasts (MCF) remodeling and proliferation. We demonstrate a novel mechanism of the Smads/miR‐675/TGFβR1 axis modulating TGFβ1‐induced MCFs remodeling and proliferation.AbstractCardiac fibroblasts (CFs) can over‐proliferate during the progression of cardiac fibrosis, accompanied by a net accumulation of extracellular matrix proteins. Based on...
Fri Apr 05, 2019 19:32
Targeting of secreted phosphoprotein 1 (SPP1) by microRNA‐340 (miR‐340) may contribute to the inhibition of proliferation, migration, invasion, and epithelial–mesenchymal transition (EMT) of gastric cancer (GC) cells via suppression of phosphatidylinositol 3‐kinase/protein kinase B (PI3K/AKT) signaling pathway.AbstractGastric cancer (GC) is a common heterogeneous disease. The critical roles of microRNA‐340 (miR‐340) in the development and progression of GC were emphasized in accumulating...
Fri Apr 05, 2019 19:32
Cord blood was collected in cord blood collection bags. CD34+ cells were then isolated using MACS system and cultured in 2D and 3D conditions for 7 days. CD34+ cell adhesion and proliferation were confirmed on polyethersulfone scaffolds. Total RNA was extracted from CD34+ cells and the miRNA signature was determined by real‐time quantitative polymerase chain reaction. Finally, the potential target genes were predicted for dysregulated microRNAs by bioinformatic tools.AbstractHuman umbilical...
Fri Apr 05, 2019 19:31
Cardiac fibroblasts treated with recombinant fibroblast growth factor 23 (FGF23) protein were characterized by an increased interaction between signal transducer and activator of transcription 3 (STAT3) and SMAD3. These results demonstrate that FGF23 induces atrial fibrosis in atrial fibrillation by increasing reactive oxygen species production and subsequently activating STAT3 and SMAD3 signaling.AbstractHigh fibroblast growth factor 23 (FGF23) concentrations are a strong predictor of atrial...
Fri Apr 05, 2019 19:31
Here we reported that human absent in melanoma 2 (AIM2) highly expressed in non‐small‐cell lung cancer (NSCLC) cells and exhibited a tumor‐promoting property both in vitro and in vivo. In addition, AIM2 functioned as an oncogene in NSCLC in an inflammasome‐dependent way.AbstractThe human absent in melanoma 2 (AIM2) is considered as a DNA recognizer. AIM2 has been described as a tumor suppressor gene in the early years. But recent studies suggested that it functions as an oncogene in several...
Fri Apr 05, 2019 19:31
Bone marrow mesenchymal stem cell‐derived conditioned medium could effectively alleviate hypoxia/reoxygenation‐induced injury of H9c2 cells. These protective effects contributed at least partially to attenuating autophagy impairment via Notch2/mTOR signaling.AbstractBone marrow mesenchymal stem cells (BMSC) can ameliorate ischemic injury of various tissues. However, the molecular mechanisms involved remain to be clarified. In this study, we intend to investigate the effects of BMSC‐derived...
Fri Apr 05, 2019 19:31
This study aims to determine the feasibility of using oligodeoxynucleotides with unmethylated cytosine‐guanine dinucleotide sequences (CpG ODN) as an immunity protection strategy for a mouse model of acute respiratory distress syndrome (ARDS). The intranasal administration of CpG ODN may be a potential treatment approach to ARDS. More studies are needed to further determine the protective mechanism of CpG ODN.AbstractThis study aims to determine the feasibility of using oligodeoxynucleotides...
Fri Apr 05, 2019 19:31
We demonstrated that C1q/TNF‐related protein‐9 improved the postmyocardial infarction early cardiac function, at least in part, by modulating M1/M2 macrophage polarization, largely via the TLR4/MD2/MyD88 and AMPK‐NF‐κB pathways.AbstractThe timely regulation of inflammatory M1 macrophage polarization toward regenerative M2 macrophages suggests the possibility of immunotherapy after myocardial infarction (MI). C1q/TNF‐related protein‐9 (CTRP9) has anti‐inflammatory effects and can ameliorate...
Fri Apr 05, 2019 19:31
The study analyzed genome‐wide DNA methylation in bladder cancer on the basis of The Cancer Genome Atlas (TCGA) data and identified differentially methylated genes (DMGs) related to bladder cancer, eventually establishing a prognostic risk model (PRM) to forecast the prognosis of bladder cancer patients.AbstractDNA methylation can regulate gene expression and is pivotal in the occurrence and development of bladder cancer. In this study, we analyzed whole‐genome DNA methylation on the basis...
Fri Apr 05, 2019 19:30
miR‐218‐5p acts as a dynamic governor during skin and hair follicle development, which may decrease the expression of SFRP2, thus promoting the transcription of β‐catenin/T‐cell factor and regulating the Wnt/β‐catenin signaling pathway.AbstractThe hair follicle is a complex biological system involved in a dynamic process governed by gene regulation. MicroRNAs play a critical role in the regulation of gene expression. We demonstrated that the expression of miR‐218‐5p and SFRP2 showed the...
Fri Apr 05, 2019 19:30
We have studied the effect of Nocturnin (NOCT) overexpression on bone and adipose tissue. We found that mice have lower fat mass which is explained by morphological changes in the size of the adipose depots. Furthermore, we provide evidence for the first time to our knowledge of NOCT localizing to the mitochondria and regulating preadipocyte mitochondrial respiration.AbstractNocturnin (NOCT) belongs to the Mg2+ dependent Exonucleases, Endonucleases, Phosphatase (EEP) family of enzymes that...
Fri Apr 05, 2019 19:30
AbstractResistance to chemotherapy represents a major cause for treatment failure in multiple myeloma (MM). Herein, this study was conducted to explore the effect of SDF‐1/CXCR4 and interleukin‐6 (IL‐6) in MM cell adhesion‐mediated chemoresistance. Enzyme‐linked immunosorbent assay was applied to detect expressions of SDF‐1α and IL‐6 in MM patients and healthy controls. RPMI‐8226 cells and isolated bone marrow stromal cells (BMSCs) were stimulated using recombinant SDF‐1α and IL‐6. Effect...
Fri Apr 05, 2019 19:30
Our present study provides the first evidence that miR‐27a‐3p/USP25 axis involves the pathogenesis of recurrent miscarriage by regulating the epithelial‐to‐mesenchymal transition program to inhibit the migration and invasion of trophoblast cells, which might provide new insights into the mechanisms underlying placental development and potential biomarkers for recurrent miscarriage.AbstractInsufficient invasion ability of trophoblasts might be associated with the development of recurrent...
Fri Apr 05, 2019 19:29
The generation of an induced pluripotent stem cell‐derived cardiomyocyte (iPSc‐CM) model from a patient with Leigh syndrome harboring the mutation m.13513G>A has revealed that this mutation impairs cardiac function, favoring a neuroectoderm commitment, in a mutant‐load dependent way.AbstractMitochondrial disorders (MDs) arise as a result of a respiratory chain dysfunction. While some MDs can affect a single organ, many involve several organs, the brain being the most affected, followed...
Thu Apr 04, 2019 19:46
In the original article, Fig. 1A was by mistakenly duplicated. The corrected image is provided in this correction article.
Fri Apr 05, 2019 03:00
Abstract α-Tubulin acetyltransferase 1 (ATAT1) catalyzes acetylation of α-tubulin at lysine 40 in various organisms ranging from Tetrahymena to humans. Despite the importance in mammals suggested by studies of cultured cells, the mouse Atat1 gene is non-essential for survival, raising an intriguing question about its real functions in vivo. To address this question, we systematically analyzed a mouse strain lacking the gene. The analyses revealed that starting at postnatal day...
Fri Apr 05, 2019 03:00
Abstract MicroRNAs (miRNAs), key regulators of gene expression at the post-transcriptional level, are grossly misregulated in some human cancers, including non-small-cell lung carcinoma (NSCLC). The aberrant expression of specific miRNAs results in the abnormal regulation of key components of signalling pathways in tumour cells. MiRNA levels and the activity of the gene targets, including oncogenes and tumour suppressors, produce feedback that changes miRNA expression levels and...
Fri Apr 05, 2019 03:00
Abstract Plakophilins (Pkp) are desmosomal plaque proteins crucial for desmosomal adhesion and participate in the regulation of desmosomal turnover and signaling. However, direct evidence that Pkps regulate clustering and molecular binding properties of desmosomal cadherins is missing. Here, keratinocytes lacking either Pkp1 or 3 in comparison to wild type (wt) keratinocytes were characterized with regard to their desmoglein (Dsg) 1- and 3-binding properties and their capability...
Thu Apr 04, 2019 03:00
SummaryA girl, born with a posterior lumbosacral giant congenital nevus, developed a central nodule that expanded over a period of 14 months into a 10 cm pedunculated mass. Histological analysis of the mass revealed melanoma of myxoid, small round‐cell type with areas of rhabdomyosarcomatous transformation confirmed by immunohistochemistry. RNA‐sequencing identified an in‐frame SASS6(e14)‐RAF1(e8) fusion in both components and the nevus. A RAF1 FISH Break‐apart test found a balanced rearrangement...
Thu Apr 04, 2019 11:00
Publication date: Available online 4 April 2019Source: Cell ReportsAuthor(s): Qin Zhang, James N. Higginbotham, Dennis K. Jeppesen, Yu-Ping Yang, Wei Li, Eliot T. McKinley, Ramona Graves-Deal, Jie Ping, Colleen M. Britain, Kaitlyn A. Dorsett, Celine L. Hartman, David A. Ford, Ryan M. Allen, Kasey C. Vickers, Qi Liu, Jeffrey L. Franklin, Susan L. Bellis, Robert J. CoffeySummaryExomeres are a recently discovered type of extracellular nanoparticle with no known biological function. Herein, we describe...
Fri Apr 05, 2019 15:11
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